Study on the structure-function relationship of polynucleotide phosphorylase: model of a proteolytic degraded polynucleotide phosphorylase
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چکیده
منابع مشابه
Study on the structure-function relationship of polynucleotide phosphorylase: model of a proteolytic degraded polynucleotide phosphorylase A.Guissani and C.Portier Institut de Biologie Physico-chimique,
It is already known that modification of E. col i polynucleotide phosphorylase by endogenous proteolysis induces drastic changes in both phosphorolysis and polymerisation reactions. The structural parameters of the proteolysed polynucleotide phosphorylase are described. The phosphorolysis of polynucleotide, which is quite progressive for the native enzyme, is shown to be only partially progress...
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The preparation of synthetic ribonucleic acid in which one of the nucleotide species is labeled with P32, makes possible studies on the distribution of the labeled nucleotide in the polynucleotide chain. Fig. 1 presents the structure of a polynucleotide in which labeled adenylic acid is randomly distributed. Hydrolysis of such a polymer with snake venom phosphodiesterase (Fig. 1A) will yield a ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1976
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/3.11.3015